Introduction
Fluorescent proteins have revolutionized biomedical research, flow cytometry, and immunoassays, providing high-precision tools for cell analysis and biomarker detection. Among them, Allophycocyanin (APC) and Cross-linked Allophycocyanin (CL-APC) stand out for their ultra-sensitive fluorescence and superior photostability. Extracted from algae, these proteins offer sustainable, high-performance alternatives to synthetic fluorophores.
Purified Allophycocyanin (APC): Ultra-Sensitive Near-Infrared Fluorescence
Allophycocyanin (APC) is a highly fluorescent phycobiliprotein that emits in the near-infrared (NIR) range, minimizing autofluorescence interference for clear, high-contrast detection. With its high quantum yield and broad emission spectrum, APC is a preferred choice for flow cytometry, fluorescence resonance energy transfer (FRET) assays, and immunoassays.
Why Choose APC ?
✅ High Quantum Yield – Delivers superior fluorescence intensity
✅ Minimal Autofluorescence – Enhances signal clarity in complex samples
✅ Ideal for Multi-Color Assays – Well-suited for flow cytometry and FRET analysis
Cross-linked Allophycocyanin (CL-APC): Enhanced Stability for Fluorescent Labeling
Cross-linked Allophycocyanin (CL-APC) is a chemically stabilized variant of APC, derived from Spirulina sp. and engineered for greater structural stability while maintaining native spectral properties. This makes CL-APC an excellent fluorophore for long-term imaging, live-cell staining, and high-sensitivity detection.
Key Benefits of CL-APC
✅ Enhanced Stability – Maintains fluorescence under varying conditions
✅ Ideal for Antibody Conjugation – Often labeled with streptavidin, primary, and secondary antibodies
✅ Reliable for Flow Cytometry & Immunofluorescence – Ensures consistent performance in biomedical assays
APC & CL-APC in Biotechnology & Medical Research
🔬 Flow Cytometry – Enables precise cell sorting and multi-color detection
🧪 Immunoassays & FRET Assays – High-sensitivity detection for biomarker research
💡 Live-Cell Imaging – Superior photostability for fluorescence microscopy
🌱 Sustainable Fluorescence Solutions – Algae-based, eco-friendly alternatives to synthetic dyes
Conclusion: The Future of Fluorescent Proteins
As the demand for high-performance, sustainable fluorophores grows, algae-derived APC and CL-APC continue to lead the way in biomedical fluorescence applications. Their high sensitivity, stability, and near-infrared emissionmake them invaluable tools for advanced research, diagnostics, and therapeutic innovations.
